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FACS and adherent cells

FACS and adherent cells - Protocols and Methods Forum

FACS and adherent cells - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-04-2006, 02:20 PM
Yuktee Dogra
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Default FACS and adherent cells



Hello,

I am looking for protocols/journal articles on the use of FACS on
adherent cells.....any help would really be appreciated!



Thank you in advance

Yuktee

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  #2  
Old 05-04-2006, 09:19 PM
J Winkler
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Default FACS and adherent cells

you must make the cells single so that they can run through sorter.


Yuktee Dogra wrote:
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  #3  
Old 05-05-2006, 02:28 AM
Stacy Ferguson
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Default FACS and adherent cells


What, specifically, are you having a problem with? Once detached,
there's no difference between analysis of cells that grow in suspension
or cells that were previously attached. The only thing you may want to
consider is that if you use trypsin to detach your cells, this may
destroy (or at least greatly reduce the number of) molecules you are
trying to detect on the surface. Using an EDTA-based detachment buffer
may be preferable.


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"Yuktee Dogra" <[Only registered users see links. ].uk> wrote:

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