| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
05-04-2006, 02:20 PM
| |||
| |||
 FACS and adherent cells Hello, I am looking for protocols/journal articles on the use of FACS on adherent cells.....any help would really be appreciated! Thank you in advance Yuktee  |
|
#2
05-04-2006, 09:19 PM
| |||
| |||
| FACS and adherent cells you must make the cells single so that they can run through sorter. Yuktee Dogra wrote: |
|
#3
05-05-2006, 02:28 AM
| |||
| |||
| FACS and adherent cells What, specifically, are you having a problem with? Once detached, there's no difference between analysis of cells that grow in suspension or cells that were previously attached. The only thing you may want to consider is that if you use trypsin to detach your cells, this may destroy (or at least greatly reduce the number of) molecules you are trying to detect on the surface. Using an EDTA-based detachment buffer may be preferable. In article <[Only registered users see links. ].net> , "Yuktee Dogra" <[Only registered users see links. ].uk> wrote: -- NewsGuy.Com 30Gb $9.95 Carry Forward and On Demand Bandwidth |
|
| Tags |
| adherent , cells , facs |
| Thread Tools | |
| Display Modes | |
|
|
Linear Mode
