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EtBr in polyacrylamide gels

EtBr in polyacrylamide gels - Protocols and Methods Forum

EtBr in polyacrylamide gels - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-29-2006, 04:37 AM
Jayakumar, R
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Default EtBr in polyacrylamide gels



Well.. I am not sure. But I can guess why. Intercalation of ethidium
bromide with DNA causes the DNA bands to slow down and may cause
mobility differences not intended. So since polyacrylamide gels are
generally used for bands of ~ 100-500 bp, this would cause a problem.
I hope I am right
Best of luck
Jayakumar

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of Emre Oktem
Sent: Friday, April 28, 2006 12:47 PM
To: [Only registered users see links. ]
Subject: EtBr in polyacrylamide gels

Hi,
We are trying to visualize DNA in polyacrylamide gels. Is there any
reason
why we shouldn't put EtBr in a polyacrylamide gel before polymerizing it
just like we do in agarose gels instead of staining it later? In the
literature I couldn't see such a technique.
I feel that there is a drawback, what can it be?

Thanks,
Emre
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