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#1
04-12-2006, 03:33 PM
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 About His-tag purification I'm expressing a HIS-tagged 16KDa protein in E.coli I got the target protein using the SDS-PAGE analysis However, the UV absorbance is 260 nm but not 280 I wander wether the protein binding with DNA/RNA in E.coli lysate How can I improve  |
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| histag , purification |
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