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About His-tag purification

About His-tag purification - Protocols and Methods Forum

About His-tag purification - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-12-2006, 03:33 PM
Zheng.Chou
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Default About His-tag purification



I'm expressing a HIS-tagged 16KDa protein in E.coli

I got the target protein using the SDS-PAGE analysis

However, the UV absorbance is 260 nm but not 280

I wander wether the protein binding with DNA/RNA in E.coli lysate

How can I improve

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