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cryopreserved primary cultured mouse cortical neurons

cryopreserved primary cultured mouse cortical neurons - Protocols and Methods Forum

cryopreserved primary cultured mouse cortical neurons - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-11-2006, 01:03 PM
Bhaswati Banerjee
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Default cryopreserved primary cultured mouse cortical neurons



Hello,

Has anyone used the commercially available cryopreserved primary cultured mouse cortical neurons for immunocytochemistry or other experiments and thereby optimized the culture conditions? If so please let me know the conditions of coating the coverslips, like what is the final dilution of Poly L lysine / Poly D Lysine used? What is the maximum yield of neurons and what are the culture conditions and anything relevant would be helpful. A detailed protocol will be highly appreciated as well.

Thanks
Bhaswati

Bhaswati Bandyopadhyay
Postdoctoral Researcher
Center for Molecular Neurobiology
1060 Carmack Rd.
Columbus, OH 43210
Tel: (614) 688-5894
Fax: (614) 292-5379
Email: [Only registered users see links. ]




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