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Ved Sharma 04-11-2006 05:04 AM

Cloudy TBS solution.
 
Thanks for your reply.

I'm using TBS for immunohistochemistry and I adjust the pH by HCl.
I never tried filtering it because when I prepare the solution, it
looks very clear. Can it be a bacterial contamination which
develops after a couple of days? Although it's hard for me to
imagine if bacteria can live on a "Tris base-NaCl" diet.

Ved

On Mon Apr 10 21:55:00 EDT 2006, "Jayakumar, R"
<[Only registered and activated users can see links. Click Here To Register...]> wrote:



Nick Theodorakis 04-11-2006 02:27 PM

Cloudy TBS solution.
 

Ved Sharma wrote:

You'd be surprised at what they grow in.

Does it smell funny when you see the turbidity? It's not always a sure
thing, but if it smells you definitely have contamination.

Nick

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Christian Praetorius 04-11-2006 02:37 PM

Cloudy TBS solution.
 
Ved Sharma <[Only registered and activated users can see links. Click Here To Register...]> wrote:


Its always astonishing in what conditions bacteria and fungi can grow
and survive. I would filter or autoclave the buffer.

Christian

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Dr Engelbert Buxbaum 04-14-2006 04:47 PM

RE: Cloudy TBS solution.
 
Ved Sharma wrote:



Probably either bacteria or fungi. Why not simply look at them in the
microscope?

By the way, keeping solutions in the fridge is good practice, as is the
addition of antibacterials like sodium azide (don't use if you detect
with peroxidase) or timerosal.


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