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-   -   Cloudy TBS solution. (http://www.molecularstation.com/forum/protocols-methods-forum/18409-cloudy-tbs-solution.html)

Jayakumar, R 04-11-2006 01:55 AM

Cloudy TBS solution.
 
You don't need to autoclave TBS if you are going to use it only for
westerns, though filtering it is a good habit. How do you adjust the
pH?? I mean what acid do you use?

Jai



-----Original Message-----
From: [Only registered and activated users can see links. Click Here To Register...]
[mailto:[Only registered and activated users can see links. Click Here To Register...].indiana.edu] On Behalf Of Ved Sharma
Sent: Monday, April 10, 2006 3:56 PM
To: [Only registered and activated users can see links. Click Here To Register...]
Subject: Cloudy TBS solution.

Hi,

I'm having trouble keeping 1X TBS solution clear/transparent. It
becomes cloudy after a couple of days. I make it by diluting 10X
TBS solution and then adjusting the pH to 7.3. I don't autoclave
after that. Is that the reason why it gets cloudy?

Thanks!

Ved

----------------------------------
Ved P. Sharma
Graduate Student
Department of Chemical Engg
University of Florida
Gainesville, FL-32611
-----------------------------------

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