Cloudy TBS solution.

Hi,

I'm having trouble keeping 1X TBS solution clear/transparent. It
becomes cloudy after a couple of days. I make it by diluting 10X
TBS solution and then adjusting the pH to 7.3. I don't autoclave
after that. Is that the reason why it gets cloudy?

Thanks!

Ved

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Ved P. Sharma
Graduate Student
Department of Chemical Engg
University of Florida
Gainesville, FL-32611
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"Ved Sharma" <vedp@ufl.edu> wrote in message
news:mailman.554.1144715304.16885.methods@net.bio. net...

Mold's I guess
Make a fresh 1x dilution each day from the 10x TBS solution , or keep the 1x
TBS in the refrigerator for a few days if that is more convenient.Keep the
1x TBS always on ice while working with it.Think of 1x TBS as milk , the
bugs like it

The mold's and other bugs don't grow in the 10x Solution because the osmotic
pressure is way to high for them.You can keep the 10x TBS on your bench at
rt. I always filter sterilise the 10x TBS and treat it with care.

hth
Gys