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5'end non-radioactive labelling

5'end non-radioactive labelling - Protocols and Methods Forum

5'end non-radioactive labelling - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-07-2006, 04:33 PM
tania lombo
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Default 5'end non-radioactive labelling



I would like to know if there is any company/method by which I can do 5'end PNK labelling with modified ATP.
I will really appreciate any suggestion.
Sincerely
Tania Lombo


Tania Bibiana Lombo Rodriguez
Graduated Student-RNA Decay and Processing
Max F. Perutz Laboratories
Departments at the Vienna Biocenter
Institute for Microbiology and Immunobiology
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  #2  
Old 04-08-2006, 03:29 AM
Austin P. So (Hae Jin)
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Default 5'end non-radioactive labelling

I think it may help if you could describe what you want to introduce.
All PNK will do is transfer the gamma phosphate of ATP onto the 5'-OH of
the ss/ds DNA.

Mind you, people then introduce a label via the 5'-Phosphate, but I have
yet to see a publication that indicates the expected yields. I think
Vector sells a kit.

Austin

tania lombo wrote:
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5end , labelling , nonradioactive


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