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tania lombo 04-07-2006 04:33 PM

5'end non-radioactive labelling
 
I would like to know if there is any company/method by which I can do 5'end PNK labelling with modified ATP.
I will really appreciate any suggestion.
Sincerely
Tania Lombo


Tania Bibiana Lombo Rodriguez
Graduated Student-RNA Decay and Processing
Max F. Perutz Laboratories
Departments at the Vienna Biocenter
Institute for Microbiology and Immunobiology
email:[Only registered and activated users can see links. Click Here To Register...].at
Dr.Bohr.G. 9, Campus Vienna Biocenter 4
A-1030
Wien, Austria
Phone: +431427754613
Fax No.+14135134634










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Austin P. So (Hae Jin) 04-08-2006 03:29 AM

5'end non-radioactive labelling
 
I think it may help if you could describe what you want to introduce.
All PNK will do is transfer the gamma phosphate of ATP onto the 5'-OH of
the ss/ds DNA.

Mind you, people then introduce a label via the 5'-Phosphate, but I have
yet to see a publication that indicates the expected yields. I think
Vector sells a kit.

Austin

tania lombo wrote:


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