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Annealing Temperatures for non-gradient themorcycle?

Annealing Temperatures for non-gradient themorcycle? - Protocols and Methods Forum

Annealing Temperatures for non-gradient themorcycle? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-31-2006, 10:17 PM
Jayakumar, R
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Default Annealing Temperatures for non-gradient themorcycle?



Well temperature would be out of question,, except otherwise you do it
one by one. But youcan still optimize Mg++ concentration in a variety
of ranges which virtually has the same effect as temperature.

Jai


Hi netters,

How can I opptimize anneling temperature in non-
gradient thermocycle (96-Well GeneAmp(r) PCR System
9700)?. Any suggestions are appreciated.

Regards

Quang

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