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Viability of Bacterial culture at -4C

Viability of Bacterial culture at -4C - Protocols and Methods Forum

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Old 03-31-2006, 05:20 AM
Siddhartha Parida
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Default Viability of Bacterial culture at -4C



Will bacterial culture stored at 30% glycerol stock be viable for 6
months to 1 yr at -4C , Generally it is mentioned to keep it at -80C

On 3/31/06, [Only registered users see links. ]
<[Only registered users see links. ].indiana.edu> wrote:


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Old 03-31-2006, 01:19 PM
Susan Hogarth
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Default Viability of Bacterial culture at -4C

Siddhartha Parida wrote:

Only one way to find out! Should be a quick experiment. My guess is
'yes', but of course it depends on the bacteria.

Hey - please don't repost an entire digest's worth fo post when asking
a question. If you use 'reply to' to aska question, at least erase all
the jnk in the digest you're replying to!

- Susan

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  #3  
Old 04-01-2006, 12:33 AM
newsnet customer
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Default Viability of Bacterial culture at -4C



Glyercol is not needed for transient storage at -4C as bacterial cultures
will not freeze at that temperature.
My guess would be for 3-6 months, and again depending on the bacteria.

ST


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