Viability of Bacterial culture at -4C

Will bacterial culture stored at 30% glycerol stock be viable for 6
months to 1 yr at -4C , Generally it is mentioned to keep it at -80C

On 3/31/06, [Only registered users see links. ]
<[Only registered users see links. ].indiana.edu> wrote:


--
Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
Bhubaneswar-3
ORISSA
09437089337(M)
[Only registered users see links. ]

Siddhartha Parida wrote:

Only one way to find out! Should be a quick experiment. My guess is
'yes', but of course it depends on the bacteria.

Hey - please don't repost an entire digest's worth fo post when asking
a question. If you use 'reply to' to aska question, at least erase all
the jnk in the digest you're replying to!

- Susan

Glyercol is not needed for transient storage at -4C as bacterial cultures
will not freeze at that temperature.
My guess would be for 3-6 months, and again depending on the bacteria.

ST