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separation of single and double strand RNA/DNA comlpex

separation of single and double strand RNA/DNA comlpex - Protocols and Methods Forum

separation of single and double strand RNA/DNA comlpex - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 03-30-2006, 04:06 PM
ali
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Default separation of single and double strand RNA/DNA comlpex



Is there any method to separate single and double stranded RNA/DNA
comples from each other(Other than hydroxyapatite columns)

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Old 04-09-2006, 06:26 AM
dr_pandu@yahoo.co.in
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Default separation of single and double strand RNA/DNA comlpex

Dear Ali,
I am not sure wjat is your RNA/DNA complex, But I have a suggetion. RNA
can be seperated from DNA using Acid phenol extraction. RNA stays in
aqueous phase. We seperate DS RNA from SSRNA by precipitating the RNA
isolated using lithium chloride.
Lithium chloride at the final concentration of 2M precipitate single
stranded RNA and the RNA present in the aqueous phage is ds RNA, which
can be precipitated by increasing the LiCl to 4M. I use this technique
to sepetrate cellular RNA (SS RNA) from the RNA of a DS RNA virus (
Bluetongue virus )
all the best
Pandu

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  #3  
Old 04-09-2006, 06:40 AM
DK
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Default separation of single and double strand RNA/DNA comlpex

In article <1144563989.907897.230440@i39g2000cwa.googlegroups .com>, [Only registered users see links. ] wrote:

And the classic technique for separating single from double stranded DNA,
DNA:RNA hybrids is cgromatography on hydroxyapatite. In terms of purity
possible to achieve, chromatography beats differential precipitation hands
down.

DK
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comlpex , double , rna or dna , separation , single , strand


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