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Annealing Temperatures for PCR

Annealing Temperatures for PCR - Protocols and Methods Forum

Annealing Temperatures for PCR - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-30-2006, 02:34 PM
Jayakumar, R
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Default Annealing Temperatures for PCR



You can calculate it using several online tools. One of my favourite is
[Only registered users see links. ]
You can also search on google with keywords "annealing temperature
calculator tool". The melting temperature is your annealing
temperature.

But still the actual Annealing temperature has tto be optimized
through gradient PCR using different conc. Off Mg and salts. The Tm
values only helps to give an idea.
Best of luck
Jayakumar

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of
[Only registered users see links. ]
Sent: Wednesday, March 29, 2006 6:31 PM
To: [Only registered users see links. ]
Subject: Annealing Temperatures for PCR

Dear sir,
From internet I know that youa an expert in Molecular Biology research.
I have a problem with my student PCR.
If you do not mind, please help me to tell me how to calculate the exact
annealing primers for pair of primers:
5'- TTA GGG CAA GAG ATG GTA AGG -3' and
5'- TTA TAA CAA TGA TGG AGG G -3'
Thank you very much for your kindness.

Best regard
Retnohh


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