Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Protocols and Methods Forum (http://www.molecularstation.com/forum/protocols-methods-forum/)
-   -   Annealing Temperatures for PCR (http://www.molecularstation.com/forum/protocols-methods-forum/18372-annealing-temperatures-pcr.html)

Jayakumar, R 03-30-2006 02:34 PM

Annealing Temperatures for PCR
 
You can calculate it using several online tools. One of my favourite is
[Only registered and activated users can see links. Click Here To Register...]
You can also search on google with keywords "annealing temperature
calculator tool". The melting temperature is your annealing
temperature.

But still the actual Annealing temperature has tto be optimized
through gradient PCR using different conc. Off Mg and salts. The Tm
values only helps to give an idea.
Best of luck
Jayakumar

-----Original Message-----
From: [Only registered and activated users can see links. Click Here To Register...]
[mailto:[Only registered and activated users can see links. Click Here To Register...].indiana.edu] On Behalf Of
[Only registered and activated users can see links. Click Here To Register...]
Sent: Wednesday, March 29, 2006 6:31 PM
To: [Only registered and activated users can see links. Click Here To Register...]
Subject: Annealing Temperatures for PCR

Dear sir,
From internet I know that youa an expert in Molecular Biology research.
I have a problem with my student PCR.
If you do not mind, please help me to tell me how to calculate the exact
annealing primers for pair of primers:
5'- TTA GGG CAA GAG ATG GTA AGG -3' and
5'- TTA TAA CAA TGA TGG AGG G -3'
Thank you very much for your kindness.

Best regard
Retnohh


_______________________________________________
Methods mailing list
[Only registered and activated users can see links. Click Here To Register...]
[Only registered and activated users can see links. Click Here To Register...]


This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.



All times are GMT. The time now is 10:48 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09255 seconds with 11 queries