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total DNA from animal skin

total DNA from animal skin - Protocols and Methods Forum

total DNA from animal skin - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 03-28-2006, 07:50 PM
Jayakumar, R
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Default total DNA from animal skin



Are you going to isolate total DNA from the animal skin tissue or from
the bacteria that lives on animal skin? It was not very clear.

Jai

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of GANESH BABU
Sent: Tuesday, March 28, 2006 9:48 AM
To: [Only registered users see links. ]
Subject: total DNA from animal skin

Hi all,
I am Ganesh Babu, PhD student at Alagappa University. I am working on
identification of bacteria associated with goat skin by 16S rRNA gene
sequencing.
Could you tell me the best method for extraction of total DNA from
animal skin for direct amplification of 16S rRNA gene for bacterial
diversity studies?
Thanking you
Ganesh Babu


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  #2  
Old 03-29-2006, 05:37 AM
3pi
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Default total DNA from animal skin

Jayakumar, R wrote:


It seems he's aiming to bacterial DNA, but maybe he needs a total DNA
extraction from the skin...


My suggestion for Ganesh is using a Guanindine/Silica DNA extraction as
described by Boom et al. (1990), refert to:

[Only registered users see links. ]

I've used it successfully to extract DNA from wild boars museum skins
(dessiccated, salt-treated ect..)

regards,
P.
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