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plasmid agarose electrophoresis

plasmid agarose electrophoresis - Protocols and Methods Forum

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Old 03-16-2006, 03:04 PM
Jayakumar, R
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Default plasmid agarose electrophoresis



Try a 6% agarose gel. A very long one preferably as big as 15 - 20 Cm
long. Prestain the gel and run them till you can observe sufficient
separation of the bands. That should do.
That should work.

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of vagos
Sent: Wednesday, March 15, 2006 3:25 PM
To: [Only registered users see links. ]
Subject: plasmid agarose electrophoresis

Hi, everyone, my name is Evagelos Liapis and I am a PhD student at the
university of Leicester. I have the following question:

I would like to separate a 4900bp from a 5014bp linear plasmid. Which
agarose would you recommend for that experiment and in what percentage?
Thank you in advance,
Evagelos Liapis

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