Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

plasmid agarose electrophoresis

plasmid agarose electrophoresis - Protocols and Methods Forum

plasmid agarose electrophoresis - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 03-16-2006, 03:04 PM
Jayakumar, R
Posts: n/a
Default plasmid agarose electrophoresis

Try a 6% agarose gel. A very long one preferably as big as 15 - 20 Cm
long. Prestain the gel and run them till you can observe sufficient
separation of the bands. That should do.
That should work.

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of vagos
Sent: Wednesday, March 15, 2006 3:25 PM
To: [Only registered users see links. ]
Subject: plasmid agarose electrophoresis

Hi, everyone, my name is Evagelos Liapis and I am a PhD student at the
university of Leicester. I have the following question:

I would like to separate a 4900bp from a 5014bp linear plasmid. Which
agarose would you recommend for that experiment and in what percentage?
Thank you in advance,
Evagelos Liapis

Methods mailing list
[Only registered users see links. ]
[Only registered users see links. ]

This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

Reply With Quote

agarose , electrophoresis , plasmid

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agarose gel electrophoresis of PCR products - Method and Protocol domba PCR - Polymerase Chain Reaction Forum 12 02-24-2012 03:39 AM
Alginate lyase experiment with agarose gel electrophoresis and assays RachBio Agarose Gel Electrophoresis Forum 0 02-07-2009 12:23 AM
Agarose DNA Gel Electrophoresis admin SDS-PAGE Gel Electrophoresis Forum 0 03-07-2008 01:13 AM
Agarose Gel Electrophoresis and DNA Fragment Purification domba Molecular Biology Articles and Protocols 0 07-31-2006 11:17 PM
plasmid agarose electrophoresis vagos Protocols and Methods Forum 2 03-15-2006 08:39 PM

All times are GMT. The time now is 11:34 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11362 seconds with 16 queries