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Question about Promoter of pBR322 ROP

Question about Promoter of pBR322 ROP - Protocols and Methods Forum

Question about Promoter of pBR322 ROP - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-09-2006, 01:02 PM
m.lumb@ucl.ac.uk
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Default Question about Promoter of pBR322 ROP



The ROP gene carries its own promoter. I transferred it to pBluescript
when I needed to lower the copy number. If you need to increase the
copy number (for instance for DNA preps) do a search for
Chloramphenicol amplification.

Mike Lumb

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