Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


UV light kills DNA contamination in PCR samples

UV light kills DNA contamination in PCR samples - Protocols and Methods Forum

UV light kills DNA contamination in PCR samples - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-19-2006, 09:58 PM
Jayakumar, R
Guest
 
Posts: n/a
Default UV light kills DNA contamination in PCR samples




If UV did not affect the single stranded PCR primers, does that also
mean that this technique can be used to eliminate and DNA contamination
in RNA preps (traditionally RNASE free DNASE is used for this purpose)?

Jai

-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of x j
Sent: Saturday, February 18, 2006 9:28 PM
To: [Only registered users see links. ]
Subject: UV light kills DNA contamination in PCR samples

Dear Sir/Maddam,

I found the following message from internet. Could you plase give me
the volumn, pages of the article that appeared on Nature. Thanks, Jun
----------------
A friend of mine found this in some lab journal, thought I'd pass it
on to you...
***
Shining UV light on PCR reagents can eliminate DNA contamination,
according to a letter in the January 4 issue of Nature. When
researchers
at the Mayo Clinic/Foundation (Rochester, MN) took PCR mix containing
between 3 and 30,000 pg of DNA, irradiated it with 254- and 300-nm bulbs
for 5 or 20 minutes, and amplified it for 30 cycles, no amplified
product
was observed. Even 3 pg of starting DNA normally gives a detectable
amount of product after 30 cycles of amplification. DNA added to an
irradiated PCR mix could still be amplified, indicating that UV
treatment
didn't affect the reagent's ability to amplify. Although a 5-minute
irradiation reduced the amplification efficiency of double-stranded DNA,
single-stranded primers in the PCR mix still maintained their integrity.
***
dan



---------------------------------
Yahoo! Autos. Looking for a sweet ride? Get pricing, reviews, & more on
new and used cars.
_______________________________________________
Methods mailing list
[Only registered users see links. ]
[Only registered users see links. ]


This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

Reply With Quote
Reply

Tags
contamination , dna , kills , light , pcr , samples


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
the absolutely final complete collection of ideas blochee Physics Forum 2 06-15-2007 06:31 AM
the absolutely final complete collection of ideas blochee Physics Forum 0 06-14-2007 10:30 PM
Moving Dimensions Theory!! Rock On!! drelliot@gmail.com Physics Forum 1 07-06-2006 05:19 PM
UV light kills DNA contamination in PCR samples x j Protocols and Methods Forum 0 02-19-2006 02:28 AM


All times are GMT. The time now is 02:23 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15588 seconds with 16 queries