Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


DNA isolation by CTAB with Proteinase-K for plant tissue?

DNA isolation by CTAB with Proteinase-K for plant tissue? - Protocols and Methods Forum

DNA isolation by CTAB with Proteinase-K for plant tissue? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-15-2006, 12:00 AM
Deanne Bell
Guest
 
Posts: n/a
Default DNA isolation by CTAB with Proteinase-K for plant tissue?



I have found that different techniques work for different plant species
depending on the presence of polysachs, phenols, oils . . . there's a
web site for a journal called Biotechniques that would be a good place
for you to start looking for different protocols.

If CTAB protoco is not giving you good quality DNA, try some of the
protocols from these authors:

Wang et al. 1996. An improved isolation of nuclear DNA from leaves of
wild grapevine dried with silica gel. Plant Mol Biol. Reporter
14:361-373

Tabaeizadeh Z., Agharbaoui Z., Harrak H., and Poysa V., (something about
transgenic tomatoes) 1999 Plant Cell Reports 19:197-202

Bernatzky & Tankley Genetics of Actin related sequences in tomato. 1986
Theor Appl Genet 72:314-321

Prince, J. something about DNA extraction from peppers.

Hope this helps,
Deanne Bell






Reply With Quote
Reply

Tags
ctab , dna , isolation , plant , proteinasek , tissue


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
DNA isolation from tissue !!SOS!! olga_m Biochemistry Forum 0 07-15-2009 10:00 AM
RNA isolation from large frozen tissue stefulj RNA Techniques Forum 5 07-06-2008 05:32 AM
RNA isolation from human adipose tissue S.Lahham@med.umcg.nl Protocols and Methods Forum 0 01-05-2007 04:05 PM
DNA isolation by CTAB with Proteinase-K for plant tissue? TRAN HO QUANG Protocols and Methods Forum 0 02-13-2006 08:30 AM
Tissue Engineering & Stem Cell Research 2005 Andrew Oliver Protocols and Methods Forum 0 12-02-2004 11:38 PM


All times are GMT. The time now is 07:27 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11399 seconds with 16 queries