We hope you had a good start in the New Year 2006 !
For your research and your qPCR experimental results we wish you all
the best !
our newsletter inform about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are summarized on the Gene Quantification
homepage. The focus in this newsletter: quantitative single-cell PCR
and the qPCR event calendar 2006
Please visit our independent Academic & Industrial Information Platform
for qPCR platform - The reference in qPCR
A lot of qPCR companies participate in our qPCR platform. On their
sub-pages new real-time cyclers, consumables, new released kits and
detection chemistries, as well as innovative amplification technologies
are presented. Links from the sub-pages will lead you to the related
product pages at the so called company homepages. For the next future
we plan additional sub-pages for independent research institutes and
laboratories (e.g. core facilities at universities, free labs, etc.)
where innovative technologies (single-cell qRT-PCR, immuno-qPCR,
micro-RNA, etc.), algorithms, calculation software and developments
around qPCR can be presented. If you are interested to join the qPCR
platform with your company or institution, please contact [Only registered users see links. ]
Single-cell molecular-biology is a relatively new scientific branch in
biology. The first single-cell analysis were involved in the
characterization of mitochondrial DNA in 1988. Single-cell DNA
analysis, in particular genomic DNA, is important and may be
informative in the analysis of genetics of cell clonality, genetic
anticipation and single-cell DNA polymorphisms. Nowadays for most
scientists the quantitative transcriptomics in a single-cell is much
more important, and the analytical method of choice is the quantitative
real-time RT-PCR. The relative abundance of single mRNAs and their up-
or down-regulation in a single cell, compared to their neighbour cells,
is the goal. The need for quantitative single-cell mRNA analysis is
evident given the vast cellular heterogeneity of all tissue cells and
the inability of conventional RNA methods, like northern blotting,
RNAse protection assay or classical block RT-PCR, to distinguish
individual cellular contributions to mRNA abundance differences.
This two pages presents interesting papers, sampling technologies and
links about single-cell qRT-PCR, using micro-manipulated or
laser-capture microdissected tissue followed by real-time RT-PCR: [Only registered users see links. ]
Limitations of mRNA amplification from small-size cell samples.
Miniaturization applied to analysis of nucleic acids in heterogeneous
Gene-expression analysis at the single-cell level.
Single-cell gene expression analysis: implications for
neurodegenerative and neuropsychiatric disorders.
Combined histochemical staining, RNA amplification, regional, and
single cell cDNA analysis within the hippocampus.
PCR amplification from single DNA molecules on magnetic beads in
emulsion: application for high-throughput screening of transcription
Single neurons as experimental systems in molecular biology.
Single-cell molecular biology: implications for diagnosis and treatment
of neurologic disease.
Methodological considerations regarding single-cell gene expression
profiling for brain injury.
Optimization of real time RT-PCR methods for the analysis of gene
expression in mouse eggs and preimplantation embryos.
Monitoring dynamics of single-cell gene expression over multiple cell
Genome amplification of single sperm using multiple displacement
Single-molecule PCR: an artifact-free PCR approach for the analysis of
Application of the real-time PCR for the detection of airborne
microbial pathogens in reference to the anthrax spores.
worldwide academic and commercial qPCR Events are listed at [Only registered users see links. ]
Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, LightCycler University, .....etc.
submit your qPCR event here [Only registered users see links. ]
TATAA Biocenter Open Course in qPCR: [Only registered users see links. ]
We have found that the demand for reliable training in the field of
quantitative real-time PCR (qPCR) is huge and to coordinate these we
aim to arrange various three day basic courses all over Europe in 2006.
The first course day will be directed to people planning or considering
using qPCR in their research and also users not yet fully familiar will
qPCR. The second day targets more advanced users and people
concentrating on different quantification strategies. The third day
focuses on aspects in sample preparation and reverse transcription. The
courses contain both theoretical seminars and a lot hands-on training
with experienced supervision. Courses in Ghent will be in collaboration
with Jo of real practical Vandesompele and all courses at TATAA
Biocenter Germany will be held under the supervision of Michael W.
=> register for TATAA workshops [Only registered users see links. ]
Course Occasions 2006:
21st - 24th February in Gothenburg, Sweden
1st - 3rd March in Freising-Weihenstephan, first workshop at TATAA
14th - 17th March in Ghent, Belgien
28th - 31st March in Gothenburg
4th - 6th April in Freising-Weihenstephan
25th - 29th April in Gothenburg
16th - 18th May in Freising-Weihenstephan
30th May - 2nd June in Gothenburg
27th - 29th June in Freising-Weihenstephan
Please send the qPCR NEWS to further scientists and friends who are
interested in qPCR and in our Academic & Industrial Information
Platform for qPCR. best regards
Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages [Only registered users see links. ]