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Question about streptomycin sulfate precipitation

Question about streptomycin sulfate precipitation - Protocols and Methods Forum

Question about streptomycin sulfate precipitation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 01-03-2006, 05:16 PM
Igal Finarov
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Default Question about streptomycin sulfate precipitation



Dear Vangelis,

I saw in Internet that you had the same question I have: what is the
percent of streptomycin sulfate needed to precipitate DNA. Can you
please share with me your knowledge on that issue.

Thank you.

Igal Finarov.

Weizmann Institute of Science.


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  #2  
Old 01-04-2006, 03:13 PM
D.K.
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Default Question about streptomycin sulfate precipitation

In article <[Only registered users see links. ].net> , "Igal Finarov" <[Only registered users see links. ].il> wrote:

Typically in the range of 0.5-1.0% final. Just make sure that it
does not precipitate your protein of interest.

DK
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  #3  
Old 01-05-2006, 11:01 AM
Duncan Clark
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Default Question about streptomycin sulfate precipitation

Historians believe that in newspost
<[Only registered users see links. ].ne t> on Tue, 3 Jan 2006,
Igal Finarov <[Only registered users see links. ].il> penned the following literary
masterpiece:

Find any 1960's or early 70's molecular biology enzyme purification
paper and you will invariably find that they used Streptomycin sulphate
pptation prior to loading their first column. Subsequently Polymin P
pptation came along. Just be very careful about pptating the protein you
may want with too much of either. You can't always back recover it.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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