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| Dear Agarwal Why do you bother about doing starch gels. About 10 years back, I performed several isozyme analyses for plant peroxidases, esterases, using native PAGE gels (no SDS anywhere). It worked out excellently. Try using PAGE gels to overcome your problems. bye and best of luck Jayakumar R. nair Dept. of Cancer Genetics Roswell park Cancer Institute Buffalo NY 14263 USA. -----Original Message----- From: [Only registered users see links. ] [mailto:[Only registered users see links. ].indiana.edu]On Behalf Of vijayendra agrawal Sent: Wednesday, December 21, 2005 12:36 PM To: [Only registered users see links. ] Subject: Starch gel Dear all, I am doing Starch gel electrophoresis in my lab for isozymis analysis of plant peroxidase. But the problem i am facing is my gel doesnot get solidified. I am using 0.3 M TBE buffer to prepare the gel. Could anyone please suggest me what to do with this stuff? Vijayendra Agrawal RLABB Maitidevi, Kathmandu Nepal _______________________________________________ Methods mailing list [Only registered users see links. ] [Only registered users see links. ] This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. |
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