This is just an idea - but I found that my 310 was very sensitive to
changes in ambient temperatures. Results would be - different molecular
weights when I ran over night vs during the day . . . I tried getting a
thermostat in the room that kept a constant temperature within 3-5
degrees F and that solved my problem. I know that there is a heating
plate for the capillary but . . . theoretical isn't always actual.
More of my 2 cents - How big are the fragments you are running? Do you
really need something 2500 bp long? I seem to recall an ABI standard
that went from 35 to 500 base pairs. Standards I bought from other
companies always had a lot of breakdown (minor noise around the 'real'
band). AND if you let standards get too old (even ABIs), they will
start breaking down.
hope this helps
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I'm using the ABI 310 to run LOH analysis for the FH gene. I'm a new user of the ABI and I keep having a problem after I run my experiment. When I try to analyze my results a message "No sizing data" is display and I don't get any peaks. If I look at the internal size standard that I'm using (GS 500) I can see some peaks but not at the expected sizes.
If anyone could shed some light on this respect, I would really appreciate it