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microsatellites problems ABI 310

microsatellites problems ABI 310 - Protocols and Methods Forum

microsatellites problems ABI 310 - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 12-14-2005, 09:15 PM
Eric P
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Default microsatellites problems ABI 310



Hello all.

We ran into a big problem running microsatellites analysis on an ABI
310. We use the 2500 size standard from ABI as an internal standard.
Running the same sample several times, we got different allele size. We
were told that the 2500 size standard was not the ideal thing to run in
denaturing conditions. We tried an other size standard (mapmarker 100)
with the same results.

We are using the GeneScan (mac version) for analysis. I was told that
the reprocibility problem as been solved on genemapper. Before bying
the thing ($$$$$$$$) I would like to know if it could really solve the
problem.

Does anyone as ever goten this reproducibility problem, and if so how
do you correct this.


Thank you for your help.


Eric Parent
Maurice Lamontagne Institute
Fisheries and Oceans Canada

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