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Coupled prokaryotic transcription/translation system

Coupled prokaryotic transcription/translation system - Protocols and Methods Forum

Coupled prokaryotic transcription/translation system - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-16-2005, 04:45 PM
Alex
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Default Coupled prokaryotic transcription/translation system



Hi all,
We are looking for a system that allows HTP generation of mkg amounts
of protein from linear PCR products. It seems that coupled prokaryotic
T7 driven is a way to go. Any suggestions or ideas? What would be the
most critical parameters? What yield to expect? Are there any
preferences among the commercial products?
Any help or advice will be greatly appreciated.
Thanks,
Alex

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  #2  
Old 11-17-2005, 10:51 AM
Christian Praetorius
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Default Coupled prokaryotic transcription/translation system

"Alex" <[Only registered users see links. ]> wrote:


I know from a coleague that there is a kit from Qiagen, which is
capable to do a coupled transcription/translation, the Qiagen
ExpressEasy Kits.

Christian

--
Immerhin ist die relative Fluechtigkeit des Mediums Computer dem Gehalt vieler Bilder
in idealer Weise angemessen, waehrend man bei Papierbildern immer ueberlegt, ob "es das
Bild wert" sei. Oliver Corff, 23.08.05 in drf
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  #3  
Old 11-17-2005, 11:30 AM
Duncan Clark
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Default Coupled prokaryotic transcription/translation system

Historians believe that in newspost
<1132159514.159552.279750@g47g2000cwa.googlegroups .com> on Wed, 16 Nov
2005, Alex <[Only registered users see links. ]> penned the following literary masterpiece:

mkg?

mg's or ug's?

From what I have heard (never used it) the highest production seems to
be using the Roche system. Maybe a look at how they do wit will give you
some ideas of what to look for.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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