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-   -   linker scanning (http://www.molecularstation.com/forum/protocols-methods-forum/18136-linker-scanning.html)

chrsjav 10-19-2005 11:18 PM

linker scanning
 
I'm an undergraduate considering a project using linker scanning
mutagenesis in Drosophila. First off, is linker scanning worth the
trouble if the lab is already going to do point (EMS) mutagenesis?

If you think it's a good idea, I'd like to maximize the information I
can obtain from the experiment. I was hoping I could get protein
structure information as well locations of regulatory sequences (the
primary goal).

Does anyone have any suggestions for kits or protocols? The GPS-LS
Linker Scanning System from NEB looks good.

My budget is about a 1000 USD.

Thanks,
Chris



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