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Off topic - Tissue Cuture issues

Off topic - Tissue Cuture issues - Protocols and Methods Forum

Off topic - Tissue Cuture issues - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 10-04-2005, 09:34 PM
Haviland, David L
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Default Off topic - Tissue Cuture issues



Hi all:

I'm trying to save the lab some money. I'm trying to diagnose some
mis-behaving tissue culture incubators that have quite a bit of water
condensation on the inside of the glass doors. Air flow motors and
filters have been replaced on all the units.

A service company keeps raising the door temperature but in reality they
raised the overall temp of the incubator killing the cells. I'm double
checking the CO2 and temp but any ideas would be appreciated.

Surely there is a simple solution here?

Thanks in advance,

David

=================================
David L. Haviland, Ph.D.
Assistant Professor, Immunology
Institute of Molecular Medicine, R907
Univ. of Texas, Houston - HSC
2121 W. Holcombe Blvd.
Houston, TX 77030
713-500-2413 - Voice
713-500-2424 - FAX
=================================

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  #2  
Old 10-05-2005, 01:28 PM
J. Winkler
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Default Off topic - Tissue Cuture issues

The approach of the support company is essentially correct - the glass
door gets too cold and you get condensation from the inside.

Increasing the door heating should do the trick, but I haven't seen
incubators where you can set the door temperature. Are you sure the door
heatings are still working at all?

Other possibilities: a warped door or rubber seals that don't seal
properly anymore -> cold air gets in -> condensation.

Haviland, David L wrote:
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