Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


red pigment in tiny insect eyes: a problem in cDNA library construction?

red pigment in tiny insect eyes: a problem in cDNA library construction? - Protocols and Methods Forum

red pigment in tiny insect eyes: a problem in cDNA library construction? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-22-2005, 04:22 PM
rini
Guest
 
Posts: n/a
Default red pigment in tiny insect eyes: a problem in cDNA library construction?



Hi,

I am isolating polyA+ RNA from the heads of a tiny insect that, like
Drosophila, has red eyes.
An intense red coloration (I suppose from the eyes) remains both in
total RNA and polyA+ RNA.
With the polyA+ RNA I succesfully did RT-PCR reactions, so I did not
worry much(however I do not know whether they would improve not having
the red pigment around).
I worry now that I possibly will use the polyA+ RNA for constructing a
cDNA library.

I used RNeasy Kit for total RNA isolation and an Ambion kit for the
polyA+ RNA purification. With following results:
total RNA:
A260/280: >1.9
A260/230: approx. 1.0
polyA+ RNA:
A260/280: approx. 1.8
A260/230: approx. 0.8
(Gel: no degradation)

Any comment?

Thanks
Rini

Reply With Quote
Reply

Tags
cdna , construction , eyes , insect , library , pigment , problem , red , tiny


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
cDNA library construction kit rini Protocols and Methods Forum 0 09-14-2005 10:32 AM
Optimal way to construct a YEAST 2-hybrid cDNA library? Apuzzlepiece@gmail.com Protocols and Methods Forum 0 08-10-2005 03:22 AM
Recommendation for cDNA library construction kit, please? Apuzzlepiece@gmail.com Protocols and Methods Forum 0 08-07-2005 12:46 AM
To solve the problem for the Cdna Construction vaniram2001@yahoo.com Protocols and Methods Forum 0 12-29-2003 07:31 AM
problem with cDNA library construction Guylaine Keller Protocols and Methods Forum 0 09-02-2003 07:38 AM


All times are GMT. The time now is 09:01 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10990 seconds with 16 queries