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strand displacement by reverse transcriptases

strand displacement by reverse transcriptases - Protocols and Methods Forum

strand displacement by reverse transcriptases - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-16-2005, 11:44 AM
rini
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Default strand displacement by reverse transcriptases



Hello,

there is some confusion regarding the meaning of the term "strand
displacement", referred to a particular feature of DNA Polymerases.
Some intend by this, the displacement (moving, dislodgement) of already
annealed DNA that the polymerase encounters while polymerising along
the DNA template. Others mean the disruption (degradation) of that
annealed DNA.

At NewEngland Biolabs I found the following definition (although they
may be wrong...):
"Strand Displacement: The term strand displacement describes the
ability to displace downstream DNA encountered during synthesis. (...)
Polymerases lacking strand displace-ment activity are used in
gap-filling reactions such as those employed in site-directed
mutagenesis protocols.
In contrast to strand displacement, some ploymerases degrade an
encountered downstream strand via a 5´->3´ exonuclease activity. This
activity is employed for nick-translation protocols."

Whatever is true, for the purpose of my question (below), I agree with
the BioLab definition.

Now my question:

Do reverse transcriptases also possess this strand displacement
activity? Looks so (see again Biolab).
However, if yes, which ones?
References?

Thanks for any suggestion.
Rini

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displacement , reverse , strand , transcriptases


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