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#1
09-15-2005, 12:48 PM
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 Gly4-Ser linker proteolytic degradation E.coli expression of a two domain protein is undergoing specific proteolytic cleavage at the (Gly4-Ser)x4 linker site between them. Tried using protease inhibitor cocktails without success. Is this a common problem? Does anyone have suggestions to what is the cause and what can be tried to prevent it? Thanks  |
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| degradation , gly4ser , linker , proteolytic |
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