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Gly4-Ser linker proteolytic degradation

Gly4-Ser linker proteolytic degradation - Protocols and Methods Forum

Gly4-Ser linker proteolytic degradation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-15-2005, 12:48 PM
patrick.stancombe@hpa.org.uk
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Default Gly4-Ser linker proteolytic degradation



E.coli expression of a two domain protein is undergoing specific
proteolytic cleavage at the (Gly4-Ser)x4 linker site between them.
Tried using protease inhibitor cocktails without success. Is this a
common problem? Does anyone have suggestions to what is the cause and
what can be tried to prevent it?
Thanks

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