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-   -   Gly4-Ser linker proteolytic degradation (http://www.molecularstation.com/forum/protocols-methods-forum/18096-gly4-ser-linker-proteolytic-degradation.html)

patrick.stancombe@hpa.org.uk 09-15-2005 12:48 PM

Gly4-Ser linker proteolytic degradation
 
E.coli expression of a two domain protein is undergoing specific
proteolytic cleavage at the (Gly4-Ser)x4 linker site between them.
Tried using protease inhibitor cocktails without success. Is this a
common problem? Does anyone have suggestions to what is the cause and
what can be tried to prevent it?
Thanks



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