The technique you describe is the preparatin of prestained agarose gels. But actually it is never added to nearly boiling agarose. The agarose is cooled till you can tolerate the temperature for 10 sec on your palm (should be about 50-60C). Add ethidium bromde stock (10 mg/ml) 1 ul for 50 ml of solution. Pour them into casting trays and allow to solidify. You can add even less if the gel fluoresces badly. Remember you cant destain prestained gels very easily.
Remember to dispose off the gel and all solutions that comes into contact with it carefully according to biohazard disposal procedures of your institute.
I hope that helps.
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[mailto:[Only registered users see links. ].indiana.edu]On Behalf Of [Only registered users see links. ]
Sent: Saturday, September 10, 2005 6:01 PM
To: [Only registered users see links. ]
Subject: ethidium bromide vapour?
A couple of people in our lab add ethidium bromide solution to hot agarose
(i.e. nearly boiling), and claim that ethidium bromide cannot escape as a
gas so there is no danger. This runs contrary to everything else I have
heard, which says the agarose solution should be left to cool first, or
the etbr added in a fume cupboard. Obviously this later method seems to be the
safest, but is there any merit to their claim?
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