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Nick translation of BAC DNA

Nick translation of BAC DNA - Protocols and Methods Forum

Nick translation of BAC DNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 09-07-2005, 12:49 PM
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Default Nick translation of BAC DNA

Dear all,
for FISH analysis I try to label BAC DNA with different
fluorochrome-labeled dUTPs by nick translation.
Normally I use a Nick Translation Mix from Roche (Cat. No. 11 745 808
910). It seems to be, that different fluorochromes are inserted with
different efficiencies (maybe because of steric reasons).
Does anyone has an idea for a better system, better enzymes?

Because of different reasons I really would like to use nick
translation and non of the other labelling methods!

Thank you very much in advance,
S. Hauke.

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bac , dna , nick , translation

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