| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
09-07-2005, 12:49 PM
| |||
| |||
 Nick translation of BAC DNA Dear all, for FISH analysis I try to label BAC DNA with different fluorochrome-labeled dUTPs by nick translation. Normally I use a Nick Translation Mix from Roche (Cat. No. 11 745 808 910). It seems to be, that different fluorochromes are inserted with different efficiencies (maybe because of steric reasons). Does anyone has an idea for a better system, better enzymes? Because of different reasons I really would like to use nick translation and non of the other labelling methods! Thank you very much in advance, S. Hauke.  |
|
| Tags |
| bac , dna , nick , translation |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| EGFP Translation Study Problem | Denise | DNA Techniques | 1 | 10-14-2011 04:23 PM |
| Describe the process of translation. Biochemistry? | katie_is_wickedd | Biochemistry Forum | 2 | 09-22-2010 05:42 AM |
| Morpholino binds RNA, relieving translation inhibition | Jon Moulton | RNA Techniques Forum | 0 | 12-12-2008 02:53 PM |
| mRNA Translation Protein Synthesis Video | molecule2005 | Protein Science | 0 | 07-30-2008 04:08 AM |
| Translation und Rotation | alexander.blessing@gmx.de | Forum Physik | 24 | 11-27-2007 08:33 PM |
Linear Mode
