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Advice on buying a 18.2Mohm, nuclease free water system. Have you got one? Are you happy with it? What are the consumable lifetimes?

Advice on buying a 18.2Mohm, nuclease free water system. Have you got one? Are you happy with it? What are the consumable lifetimes? - Protocols and Methods Forum

Advice on buying a 18.2Mohm, nuclease free water system. Have you got one? Are you happy with it? What are the consumable lifetimes? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 09-06-2005, 03:35 PM
kh
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Default Advice on buying a 18.2Mohm, nuclease free water system. Have you got one? Are you happy with it? What are the consumable lifetimes?



Hello,

(Apologies if you get this twice - I tried posting via methods at
net.bio.net on Sunday, but it still hasn't gone through)

I am in the process of buying a lab water purification system capable
of producing 18.2Megohm.cm nuclease-free water. Below are all the
systems I have found that can do this.

But I have found very little information on how the brands compare,
either technically (frequency of breakdowns/faults, ease of use), or
economically (replacement cartridge/filter costs and lifetimes).

So, if anyone has used any any of the systems below (or anything else
meeting the above criteria), I'd be very grateful if you could tell me:

1. Which system?

2. What typical lifetimes have you found for each of the replaceable
cartridges and filters? (In litres of water if you know it, or else
approx. number of months)

3. Have you had any notable problems with the system?

4. If you have used any of the other systems also - how would you
compare this one with them?

Hopefully this thread could become a good resource for anyone else
looking for this type of water system.

Best regards,

Ken,

Cork,
Ireland.

--------
(For some reason, Barnstead only list their systems with both
ultrafiltration (UF) and a UV lamp as capable of removing nucleases,
while everyone else says their plain UF systems can do it. They all
have models with a UV lamp also, but recommend these where you need to
minimize the organic carbon in the water).

--
Barnstead Easypure II UV/UF
[Only registered users see links. ]

Consumables
1. Ultrafilter
2. Pretreatment cartridge
3. EASYpure ULTRApure cartridge
4. EASYpure high purity/low TOC cartridge
5. 0.2um final filter
6. UV lamp

--
Barnstead NANOpure Diamond Life Science
[Only registered users see links. ]

Consumables
1. Ultrafilter
3. Cartridge pack
5. 0.2um final filter (Manual says replace at least every 30-days)
6. UV lamp

--
Millipore Milli-Q Biocel
[Only registered users see links. ]

Consumables
1. Q-gard cartridge (pretreatment)
2. Quantum cartridge (ionic and organic removal)
3. Pyrogard 5000 UF cartridge (ultrafiltration)
4. 0.2um final filter

--
Satrorius Arium 611UF
[Only registered users see links. ]

Consumables
1. Cartridge kit
2. Ultrafiltration cartridge
3. 0.2um final filter
4. Sanitization kit

--
Labconco WaterPro PS/UF
[Only registered users see links. ]

Consumables
1. Cartridge kit (1x carbon, 3x deionization)
2. ultrafilter
3. final filter
[Detailed cartridge lifetimes given in manual. The worst case they
list is
100us/cm feed water, which gives a cartridge lifetime of 1775
litres]

--
USFilter/ELGA Purelab Ultra Genetic or Bioscience
[Only registered users see links. ]

Consumables
1. Cartridge pack
2. Ultrafilter
3. 0.2um point-of-use filter
4. Sanitization tablets

--
APS? Aquamax UF
[Only registered users see links. ]

Consumables
1. HP-Pack (pretreatment/ionic removal catridges)
2. Ultrafilter
3. 0.2um final filter

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  #2  
Old 09-07-2005, 02:47 PM
Dr Engelbert Buxbaum
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Default Advice on buying a 18.2Mohm, nuclease free water system. Have you got one? Are you happy with it? What are the consumable lifetimes?

kh wrote:


The only system I have experience with is the combination of a MiliRho
reversed osmosis system for "single distilled" and a MiliQ Ultrapure
water supply (both Milipore). That's certainly not the cheapest
solution, but I never had problems either.

The water from the MiliRho can be used for all the general clean water
needs in a fairly big lab (like rinse cycles in dish washers). When fed
with the pre-purified water, the cartridges of the MiliQ last longer. So
we simply take the cartridge out and put it back in, effectively running
it twice as long as recommended by the manufacturer.
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