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FW: amazonian plant DNA extraction
Here's 3 of my favorite techniques for "difficult" plants, of course I
modified the techniques to do microtube preps!
I was wondering how are you grinding your tissue, as that can make a big
difference in how much DNA you get.
1) J.P. Prince et al. 1997 Hort Science 32(5) pp 937-939 (good
for plants high in oils)
2) Wang, Wang & Zou. 1996 "An Improved Procedure for the isolation
of nuclear DNA from leaves of wild grapevine dried with silica gel"
? 14(4) pp 369 - 373 (first buffer breaks cell wall and isolates
nucleas, second buffer releases nucleic acids; good on fresh tissues
high in phenolics)
3) Bernatzky and Tanksley. 1986 Theor Appl Genet 72: pp 314-321 - I
have modified this protocol quite a bit, but that is where i started.
I also remember someone publishing a protocol in BioTechniques on DNA
extraction from Rain Forest Plant Species. Another technique that may
be helpful was in Plant Molecular Biology Reporter on DNA isolation from
Sedum - i remember the phrase 'highly mucilagenous succulent plant' in
the title. Both of these would have been between 1996 and 1999.
hope this helps
USDA Agricultural Research Service
Crop Diseases, Pests & Genetics
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Parlier, CA 93648
voice (559) 596-2806
fax (559) 596-2897
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|amazonian , dna , extraction , plant|
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