Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


FW: amazonian plant DNA extraction

FW: amazonian plant DNA extraction - Protocols and Methods Forum

FW: amazonian plant DNA extraction - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-23-2005, 05:47 PM
Deanne Bell
Guest
 
Posts: n/a
Default FW: amazonian plant DNA extraction






Hi Lorena
Here's 3 of my favorite techniques for "difficult" plants, of course I
modified the techniques to do microtube preps!
I was wondering how are you grinding your tissue, as that can make a big
difference in how much DNA you get.


1) J.P. Prince et al. 1997 Hort Science 32(5) pp 937-939 (good
for plants high in oils)

2) Wang, Wang & Zou. 1996 "An Improved Procedure for the isolation
of nuclear DNA from leaves of wild grapevine dried with silica gel"
? 14(4) pp 369 - 373 (first buffer breaks cell wall and isolates
nucleas, second buffer releases nucleic acids; good on fresh tissues
high in phenolics)

3) Bernatzky and Tanksley. 1986 Theor Appl Genet 72: pp 314-321 - I
have modified this protocol quite a bit, but that is where i started.


I also remember someone publishing a protocol in BioTechniques on DNA
extraction from Rain Forest Plant Species. Another technique that may
be helpful was in Plant Molecular Biology Reporter on DNA isolation from
Sedum - i remember the phrase 'highly mucilagenous succulent plant' in
the title. Both of these would have been between 1996 and 1999.


hope this helps
Deanne Bell
USDA Agricultural Research Service
Crop Diseases, Pests & Genetics
9611 South Riverbend Avenue
Parlier, CA 93648
voice (559) 596-2806
fax (559) 596-2897
[Only registered users see links. ]




Reply With Quote
Reply

Tags
amazonian , dna , extraction , plant


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Job Opening: Plant Ontology Project Coordinator Pankaj Jaiswal (OSU) Arabidopsis and Plant Biology 0 06-16-2009 05:44 PM
Plant Protein Extraction the Hard Way danfive Protein Science 0 04-24-2008 08:53 PM
Plant Science positions at the University of Nebraska Julie M. Stone Arabidopsis and Plant Biology 0 11-17-2007 01:36 PM
4 Post doc positions within Plant Biology at the University ofCopenhagen Søren Bak Arabidopsis and Plant Biology 0 03-25-2007 08:52 PM
amazonian plant DNA extraction Lorena Quintero Barrera Protocols and Methods Forum 0 08-22-2005 02:22 PM


All times are GMT. The time now is 05:28 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12232 seconds with 16 queries