I need to stain polylisine - free and conjugated with peptide. Coomasie
Brillant Blue fails with free polylysine and - for my suprise - so does
I'm using silver staining method from Amersham Technical Manual (as it say -
based on Heukeshoven and Dernick 1985) but they do not describe which
aminoacids are essential for detection.
What is the molecular weight of your peptides? If it is too small, they
will be eluted from the gel during the staining procedure. Cross-linking
with glutardialdehyde may help in this case.
Also, polylysine will have a considerable positive charge, which would
interfere with Ag+ binding. Staining with an amine reactive fluorescent
probe may be the preferred option, either before or after
electrophoresis. Check the Molecular Probes catalog ([Only registered users see links. ]) for