[Methods-and-reagents] Sequencing Difficulties

Dear Zhonglin,

seems that your sequencing rxn gets trapped at the hairpin. You might try
to add denaturing stuff like betain, DMSO, dITP etc etc like we did in the
good (?) old (!) days of manual sequencing.

Wo






Bochum University Hospital "Bergmannsheil"
- the world's first casualty hospital for miners and steel workes -
Germany

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