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[Methods-and-reagents] Sequencing Difficulties

[Methods-and-reagents] Sequencing Difficulties - Protocols and Methods Forum

[Methods-and-reagents] Sequencing Difficulties - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-24-2005, 09:23 AM
Wofo
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Default [Methods-and-reagents] Sequencing Difficulties



Dear Zhonglin,

seems that your sequencing rxn gets trapped at the hairpin. You might try
to add denaturing stuff like betain, DMSO, dITP etc etc like we did in the
good (?) old (!) days of manual sequencing.

Wo






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