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|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
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[Methods-and-reagents] Sequencing Difficulties
seems that your sequencing rxn gets trapped at the hairpin. You might try
to add denaturing stuff like betain, DMSO, dITP etc etc like we did in the
good (?) old (!) days of manual sequencing.
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|difficulties , methodsandreagents , sequencing|