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pMS119EH map and sequence

pMS119EH map and sequence - Protocols and Methods Forum

pMS119EH map and sequence - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 06-22-2005, 03:43 PM
Jennifer L. DuBois
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Default pMS119EH map and sequence



Hi there,

I have precisely the same question as someone posed back in 1994. I am looking
for a sequence and map of plasmid pMS119EH, based on pJF119EH by Furste et al.
Does anyone know where such information can be obtained?

Thanks,
Jen
--
Jennifer L. DuBois
Assistant Professor of Chemistry
265 Stepan Hall of Science
University of Notre Dame
Notre Dame, Indiana 46556
phone: 574-631-2696






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  #2  
Old 06-23-2005, 11:30 AM
Jerry M.
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Default pMS119EH map and sequence

On Wed, 22 Jun 2005 10:43:23 -0500, "Jennifer L. DuBois"
<[Only registered users see links. ]> wrote:


Hi Jen,

pMS119EH is simply a NruI-NdeI deletion in pJF119EH (Strack et al.
1992 J.Biol.Chem. 267:13062-13072). This paper is freely available
([Only registered users see links. ]).

pJF119EH, as you know, is fully described in Furste et al, with maps
(of the very similar parental plasmid pJF118EH) and all.

To the best of my knowledge, nobody has ever assembled the resulting
deduced sequences, but this should be relatively straightforward to
do.

Good luck,

JM
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