Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Quantitation of phage DNA - EtBr vs OD260

Quantitation of phage DNA - EtBr vs OD260 - Protocols and Methods Forum

Quantitation of phage DNA - EtBr vs OD260 - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-09-2005, 06:13 PM
Deanne Bell
Guest
 
Posts: n/a
Default Quantitation of phage DNA - EtBr vs OD260




** Do free nucleotides absorb at OD260? I really
**should know that shouldn't I?
**
**Chris

Hi again
As Raymond pointed out earlier
**>Remember that OD260 is measuring everything that absorbs at 260 nm
**>which includes RNA and degraded DNA etc.
I think there are things other than just nucleotides that can affect
A260/280. Any type of purification (short of cesium) will have some
kind of contaminants. We just don't care about them unless they
interfere with processes up stream.
Cheers,
Dee
---
Reply With Quote
Reply

Tags
dna , etbr , od260 , phage , quantitation


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
prevent EtBr from moving AndyFrey Protocols and Methods Forum 15 11-16-2010 09:56 AM
Phage Display Woes hubahopp@gmx.de Protocols and Methods Forum 4 12-17-2005 02:52 AM
Quantitation of phage DNA - EtBr vs OD260 C Coward Protocols and Methods Forum 3 06-10-2005 06:38 AM
Quantitation of phage DNA - EtBr vs OD260 Deanne Bell Protocols and Methods Forum 0 06-09-2005 02:56 PM
Double band in phage CsCl gradient prep C Coward Protocols and Methods Forum 5 02-01-2005 08:55 AM


All times are GMT. The time now is 12:14 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12020 seconds with 16 queries