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Quantitation of phage DNA - EtBr vs OD260

Quantitation of phage DNA - EtBr vs OD260 - Protocols and Methods Forum

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Old 06-09-2005, 02:56 PM
Deanne Bell
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Default Quantitation of phage DNA - EtBr vs OD260



**-----Original Message-----
**Subject: Re: Quantitation of phage DNA - EtBr vs OD260
**C Coward wrote:
**> I have prepared some bacteriophage DNA but I'm suspicious of my
**> quantitation of it.
*> Chris


**I always trust ethidium staining.
**
**Raymond

~*~*~*~*~*~*~*
Hi Chris
IMHO: I think you have a very good question about differences in
abiltiy to intercalate, but I agree with Raymond and always trust my
EtBr staining over the A260/280.
HTH, Dee
---
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dna , etbr , od260 , phage , quantitation


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