My teaching philosophy is to have the students making & pouring their
own gels as part of the practical!
I believe that they should also be making their own buffers - teacher
supplies the recipes (i.e. 375 mM Tris.HCl, pH 8.8, 0.1% SDS) but they
need to have experience practically mauvering in a lab.
that's my 2 cents worth, be it ever so humble
**From: [Only registered users see links. ]
**[email@example.com] On Behalf Of Michael D Jones
**Sent: Tuesday, June 07, 2005 10:28 AM
**To: [Only registered users see links. ]
**Subject: Commercial Premade SDS protein gels
**I run a practical for MSc students, and after 9 years am tied
**of making 12% SDS PAGE gels for them!
**Can anyone recommend a UK supplier of premade SDS gels.
**I generally make 12% PAGE (375 mM Tris.HCl, pH 8.8, 0.1% SDS)
**with a 4% stack (125 mM Tris.HCl, pH 6.8, 0.1% SDS).
**The gel tanks we use are the SE 250 which take 10 x 8 cm
**gels, 0.75 mm thick.
**Any advice and comments gratefully received.
**Department of Infectious Diseases
**Division of Investigative Science
**Faculty of Medicine
**Imperial College London
**Email: [Only registered users see links. ]
Historians believe that in newspost
<DBEC634514555246BBD3858755BA3C372B0044@server04.f resno.ars.usda.gov> on
Tue, 7 Jun 2005, Deanne Bell <[Only registered users see links. ].usda.gov> penned the
following literary masterpiece:
Sorry Mick, but I'm afraid I tend to agree with Deanne for an MSc
I take sandwich students every year (from a highly rated university) and
their knowledge of how to make buffers up, or even calculate amounts
from a recipe, could be written in one word - none! Suffice to say when
they leave after a year they can do this and a lot more, including their
own PAGE gels. I know this just isn't being taught in practicals any
more, what few practicals there are now. It's a basic skill and my pet
hate about degree courses nowadays.
Anyway I'm guessing there is insufficient time in the practicals for
them to make the buffers, pour the gels and do the experiment :-(
9 years of doing it for them and you deserve a break!
As to who to buy from? The only one that comes to mind is
Invitrogen/Novex. Must be others though?
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
For what it's worth, I just got an ad from somebody called Elchrome
Scientific who are trying to sell precast gels ("blotex") which are not
based on acrylamide, but on an unspecified "hydrogel", allegedly
non-toxic. For student practicals that could be worth a shot. They also
claim superior recovery during western blotting compared to standard
Other than that, BioRad sells precasts and IIRC Pharmacia (now part of