Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Commercial Premade SDS protein gels

Commercial Premade SDS protein gels - Protocols and Methods Forum

Commercial Premade SDS protein gels - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-07-2005, 05:52 PM
Deanne Bell
Guest
 
Posts: n/a
Default Commercial Premade SDS protein gels



My teaching philosophy is to have the students making & pouring their
own gels as part of the practical!
I believe that they should also be making their own buffers - teacher
supplies the recipes (i.e. 375 mM Tris.HCl, pH 8.8, 0.1% SDS) but they
need to have experience practically mauvering in a lab.

that's my 2 cents worth, be it ever so humble
Deanne Bell





**-----Original Message-----
**From: [Only registered users see links. ]
**[mailtowner-methods@hgmp.mrc.ac.uk] On Behalf Of Michael D Jones
**Sent: Tuesday, June 07, 2005 10:28 AM
**To: [Only registered users see links. ]
**Subject: Commercial Premade SDS protein gels
**
**Hi
**
**I run a practical for MSc students, and after 9 years am tied
**of making 12% SDS PAGE gels for them!
**
**Can anyone recommend a UK supplier of premade SDS gels.
**
**I generally make 12% PAGE (375 mM Tris.HCl, pH 8.8, 0.1% SDS)
**with a 4% stack (125 mM Tris.HCl, pH 6.8, 0.1% SDS).
**
**The gel tanks we use are the SE 250 which take 10 x 8 cm
**gels, 0.75 mm thick.
**
**Any advice and comments gratefully received.
**
**Mick
**
**
**Mick Jones
**Department of Infectious Diseases
**Division of Investigative Science
**Faculty of Medicine
**Imperial College London
**Email: [Only registered users see links. ]
**
**---
**
---
Reply With Quote
  #2  
Old 06-08-2005, 09:49 AM
Duncan Clark
Guest
 
Posts: n/a
Default Commercial Premade SDS protein gels

Historians believe that in newspost
<DBEC634514555246BBD3858755BA3C372B0044@server04.f resno.ars.usda.gov> on
Tue, 7 Jun 2005, Deanne Bell <[Only registered users see links. ].usda.gov> penned the
following literary masterpiece:

Sorry Mick, but I'm afraid I tend to agree with Deanne for an MSc
course.

I take sandwich students every year (from a highly rated university) and
their knowledge of how to make buffers up, or even calculate amounts
from a recipe, could be written in one word - none! Suffice to say when
they leave after a year they can do this and a lot more, including their
own PAGE gels. I know this just isn't being taught in practicals any
more, what few practicals there are now. It's a basic skill and my pet
hate about degree courses nowadays.

Anyway I'm guessing there is insufficient time in the practicals for
them to make the buffers, pour the gels and do the experiment :-(

9 years of doing it for them and you deserve a break!

As to who to buy from? The only one that comes to mind is
Invitrogen/Novex. Must be others though?

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
Reply With Quote
  #3  
Old 06-13-2005, 06:54 PM
Dr Engelbert Buxbaum
Guest
 
Posts: n/a
Default Commercial Premade SDS protein gels

Duncan Clark wrote:



For what it's worth, I just got an ad from somebody called Elchrome
Scientific who are trying to sell precast gels ("blotex") which are not
based on acrylamide, but on an unspecified "hydrogel", allegedly
non-toxic. For student practicals that could be worth a shot. They also
claim superior recovery during western blotting compared to standard
gels.

Other than that, BioRad sells precasts and IIRC Pharmacia (now part of
GE) too.
Reply With Quote
Reply

Tags
commercial , gels , premade , protein , sds


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 12:46 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.18162 seconds with 16 queries