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FW: thermal cycler preferences

FW: thermal cycler preferences - Protocols and Methods Forum

FW: thermal cycler preferences - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 05-03-2005, 03:01 PM
Deanne Bell
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Default FW: thermal cycler preferences

We have an eppendorf master cycler.
I have found the gradient capabilities invaluable to optimize annealing
temps - which vary from machine to machine, lab to lab, and for
different enzymes.
No affiliation, just like the machine
Deanne Bell

**-----Original Message-----
**From: [Only registered users see links. ]
**[mailtowner-methods@hgmp.mrc.ac.uk] On Behalf Of "Michael
**L. Sullivan"
**Sent: Monday, May 02, 2005 12:16 PM
**To: [Only registered users see links. ]
**Subject: thermal cycler preferences
**I'm planning on buying an additional thermal cycler for my
**lab and was hoping for some additional input from members of
**this forum.
**Currently we use an ABI GeneAmp 9700. Replacing that is an
**option, or opting for their lower end model, the 2720 . The
**2720 costs less, so I could think about getting two to
**address capacity issues). Since we mostly just use 0.2 ml
**tubes/striptubes, the lower end model seems like it would suffice.
**Another option would be an MJ DNA engine (now part of
**Bio-Rad). I have had good luck with these units when I was a
**post doc, and the flexibility of the unit seems really nice.
**The 96-well block can have a temp gradient across the block
**(sounds useful for optimization).
**They also have a dual 48-well block that essentially makes it
**into two independent instruments. This seems attractive as we
**may have more people wanting to use the instrumentation in
**the coming years.
**I'd be interested to hear opinions about these or other
**comparable instruments, especially with respect to
**reliability, quality, ease of use, and value.
**Thanks all.
************************************************** ******
**Please note new phone and fax numbers below
************************************************** ******
**Michael L. Sullivan, Ph.D
**Research Plant Molecular Geneticist
**U.S. Dairy Forage Research Center
**1925 Linden Drive West
**Madison WI, 53706
**(608) 890-0046 Phone
**(608) 890-0076 Fax
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