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How do His-Tag In-Gel stains work?

How do His-Tag In-Gel stains work? - Protocols and Methods Forum

How do His-Tag In-Gel stains work? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-21-2005, 02:26 PM
peter mcandrew
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Default How do His-Tag In-Gel stains work?



Hello All,

I was wondering if anybody had any ideas how the in-gel staining kits for
His-Tag proteins work. I'm keen on the time saving aspect but less so on
the price.

Cheers

Craig

---
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Old 04-22-2005, 12:18 AM
DK
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Default How do His-Tag In-Gel stains work?

In article <[Only registered users see links. ].clara.net>, [Only registered users see links. ] ("peter mcandrew") wrote:


I don't really know but just a guess:

Could it be some lanthanides? I seem to recall a specific chemilumincescence
after binding to Ca-binding proteins (e.g. complexation). Simialr complexation
would ocur with the 6His thus enabling "specific" his-tag detection.

DK
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