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Sephadex columns for nucleic acids clean up

Sephadex columns for nucleic acids clean up - Protocols and Methods Forum

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  #1  
Old 04-06-2005, 09:20 PM
Jill of all trades
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Default Sephadex columns for nucleic acids clean up



Way back when, a company called 5 prime -> 3 prime sold a small
sephadex column. It was used for DNA or RNA purfication subsequent to
various enzymatic reactions. The column was ready-to-use (no prep
necessary, just uncap and drain) and was designed for use in a
centrifuge/microfuge. 5 prime -> 3 prime is no longer in business and
I've been trying to find someone else who sells these things. Does
anyone know if they are still out there somewhere?
I know I can make my own. I know I can use columns that aren't
designed for a centrifuge. I know there are columns and strategies
that use something other than sephadex but right now I'm not
interested in those options, just the ready to go sephadex columns.

Thanks so much!
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  #2  
Old 04-07-2005, 12:17 AM
Ben Long
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Default Sephadex columns for nucleic acids clean up

Jill,

Essentially any Sephadex G10 type of column will give you useful and quick
desalting/buffer exchange for nucleic acids if they are above about 20 kDa
in size. If this is a problem, check out The Nest Group
([Only registered users see links. ]) who supply a bucket load of columns for all sorts
of uses. They take orders on-line and delivery is quick. I am in Australia
and have had them deliver to me from the US east coast within a week!
E-mail Amos there for specific info and protocols.

Cheers,

Ben


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  #3  
Old 04-07-2005, 01:57 AM
Louis Hom
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Default Sephadex columns for nucleic acids clean up

I used to get miniature gel filtration spin columns from Princeton
Separation Products.
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  #4  
Old 04-07-2005, 08:40 AM
Oliver Berkowitz
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Default Sephadex columns for nucleic acids clean up

Hi,

Amersham sells minicolumns (G25, G50)

Cheers

Oliver

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  #5  
Old 04-07-2005, 08:28 PM
GysdeJongh
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Default Sephadex columns for nucleic acids clean up


"Jill of all trades" <[Only registered users see links. ]> wrote in message
news:ea69d65c.0504061320.6d6ff9f5@posting.google.c om...

Hi,
we used Sephacryl columns from Pharmacia , then Pharmacia Biotech , then
Amersham Biosciences now GE Healthcare look here :

[Only registered users see links. ]

hth
Gys

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