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SDS-PAGE band quantification

SDS-PAGE band quantification - Protocols and Methods Forum

SDS-PAGE band quantification - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 03-23-2005, 12:38 AM
Ben Long
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Default SDS-PAGE band quantification



Hi all,

Can anyone recommend a good method for reliable protein quantification of
bands on SDS-PAGE gels. Info on appropriate stain and visualisation
techniques needed.

Many thanks,

Ben
[Only registered users see links. ]


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  #2  
Old 04-01-2005, 09:05 PM
Kathleen
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Default SDS-PAGE band quantification

We use NIH imaging software. It is meant for a Mac, but there is
version for PC's at [Only registered users see links. ]. When quantifying the amount of
protein we always include a sample of known concentration on our gel.
It works for both Coomassie blue stained gels and for western blot
films.
kathleen

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  #3  
Old 04-01-2005, 09:05 PM
Kathleen
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Default SDS-PAGE band quantification

We use NIH imaging software. It is meant for a Mac, but there is
version for PC's at [Only registered users see links. ]. When quantifying the amount of
protein we always include a sample of known concentration on our gel.
It works for both Coomassie blue stained gels and for western blot
films.
kathleen

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  #4  
Old 04-01-2005, 09:05 PM
Kathleen
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Default SDS-PAGE band quantification

We use NIH imaging software. It is meant for a Mac, but there is
version for PC's at [Only registered users see links. ]. When quantifying the amount of
protein we always include a sample of known concentration on our gel.
It works for both Coomassie blue stained gels and for western blot
films.
kathleen

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  #5  
Old 04-02-2005, 01:20 AM
Jose de las Heras
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Default SDS-PAGE band quantification


"Kathleen" <[Only registered users see links. ].edu> wrote in message
news:1112389526.789371.57460@z14g2000cwz.googlegro ups.com...

I agree with that. I use Scion Image for all our quantitation needs (DNA,
RNA and protein). Very good, very simple to use... and free.

Jose


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  #6  
Old 01-25-2011, 07:03 AM
Pipette Filler
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Default Re: SDS-PAGE band quantification

Can anyone give me a detailed analysis method for the SDS-PAGE protein bands with Scion image.

Thanks
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