bacteria mating on agar beter than in solution？

I don't know how to find books which about the bacteria mating on
agar, so please told me some books or webs to find my
question.........thanks a lot～

I don't know a book reference, but the method that has worked best for
me is to mix the donor and recipient and pass them through a 0.22 uM
filter that's in a screw-apart cartridge. Then you take the filter and
put it bacteria side up on an agar plate and grow overnight.

Scooter the Mighty wrote:


Same here. I don't know about books. I just mix both freshly grown donor
and receptor strains (sometimes also a helper strain) in 50 ul of NaCl
0.9% and dispense the mix on a 0.22 filter already placed on the agar
plate. I incubate the plate face up, overnight, take the filter, wash
the cells in NaCl 0.9% and plate an aliquot on the selective media. It
works great.
Sometimes, when I don't have filters, I just drop the mix directly on
the agar. Next day I collect the cells, wash and plate on the selective
media. I haven't seen important differences in efficiency... it always
works great.
Sergio