bacteria mating on agar beter than in solution？
 

I don't know how to find books which about the bacteria mating on
agar, so please told me some books or webs to find my
question.........thanks a lot～

bacteria mating on agar beter than in solution？
 

I don't know a book reference, but the method that has worked best for
me is to mix the donor and recipient and pass them through a 0.22 uM
filter that's in a screw-apart cartridge. Then you take the filter and
put it bacteria side up on an agar plate and grow overnight.

bacteria mating on agar beter than in solution？
 

Scooter the Mighty wrote:


Same here. I don't know about books. I just mix both freshly grown donor
and receptor strains (sometimes also a helper strain) in 50 ul of NaCl
0.9% and dispense the mix on a 0.22 filter already placed on the agar
plate. I incubate the plate face up, overnight, take the filter, wash
the cells in NaCl 0.9% and plate an aliquot on the selective media. It
works great.
Sometimes, when I don't have filters, I just drop the mix directly on
the agar. Next day I collect the cells, wash and plate on the selective
media. I haven't seen important differences in efficiency... it always
works great.
Sergio