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-   -   bacteria mating on agar beter than in solution? (http://www.molecularstation.com/forum/protocols-methods-forum/17921-bacteria-mating-agar-beter-than-solution-65311%3B.html)

Stephen 03-19-2005 06:17 AM

bacteria mating on agar beter than in solution?
 
I don't know how to find books which about the bacteria mating on
agar, so please told me some books or webs to find my
question.........thanks a lot~

Scooter the Mighty 03-24-2005 11:33 PM

bacteria mating on agar beter than in solution?
 
I don't know a book reference, but the method that has worked best for
me is to mix the donor and recipient and pass them through a 0.22 uM
filter that's in a screw-apart cartridge. Then you take the filter and
put it bacteria side up on an agar plate and grow overnight.


Sergio 03-25-2005 02:20 AM

bacteria mating on agar beter than in solution?
 
Scooter the Mighty wrote:


Same here. I don't know about books. I just mix both freshly grown donor
and receptor strains (sometimes also a helper strain) in 50 ul of NaCl
0.9% and dispense the mix on a 0.22 filter already placed on the agar
plate. I incubate the plate face up, overnight, take the filter, wash
the cells in NaCl 0.9% and plate an aliquot on the selective media. It
works great.
Sometimes, when I don't have filters, I just drop the mix directly on
the agar. Next day I collect the cells, wash and plate on the selective
media. I haven't seen important differences in efficiency... it always
works great.
Sergio


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