Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Expressing a tpeptide

Expressing a tpeptide - Protocols and Methods Forum

Expressing a tpeptide - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-14-2005, 12:22 PM
Emilie
Guest
 
Posts: n/a
Default Expressing a tpeptide



Following my problems at reamplifying a full-length candidate gene I
am interested in, my boss suggested to amplify only a "portion" [2.5
KB cDNA] of it, containing all the conserved regions, and giving up on
the 3'end which is not really conserved.
The PCR was successfull, and before moving on the cloning and
expression steps of the work, I was wondering if this approach was
likely to be succesfull. I thought it was necessary to have the
complete gene sequence for a good folding of the encoded protein, and
an active heterologous protein. Does anybody have experience in this
domain?
Reply With Quote
  #2  
Old 03-14-2005, 01:08 PM
D.K.
Guest
 
Posts: n/a
Default Expressing a tpeptide

In article <[Only registered users see links. ]> , [Only registered users see links. ] (Emilie) wrote:

One of the definitions of "protein domain" is "autonomous folding
unit". These are sometimes very hard to predict accurately.
Even if what you clone is a true individual domain of your protein,
it may not fold in E.coli (many possible reasons).

What your boss suggested is perfectly reasonable but if it
fails it means little.

DK
Reply With Quote
Reply

Tags
expressing , tpeptide


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Fixing and Preserving GFP Expressing Cells darkwng Immunofluorescence Forum 4 01-21-2010 05:50 PM
Plasmid available for expressing only TAP-tag as control? Linnea Hedin Yeast Forum 0 07-17-2008 09:31 AM
Stable cell line constitutively expressing GFP-tagged protein Ph Sh Protocols and Methods Forum 2 07-20-2006 01:32 PM
What volume to use when expressing and purifying a protein? J T Protocols and Methods Forum 2 05-17-2006 08:54 AM
Expressing an Archaeal DNA polymerase in E. coli Damien Protocols and Methods Forum 1 10-21-2003 11:45 AM


All times are GMT. The time now is 06:32 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11434 seconds with 16 queries