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| Hi there I got the library from ABRC for Y2H. In the kit there are four Ecoli strains. Does anybody know which antibiotics I should add if I want to growth those bacterial strains (pAS1, pAS1-CYH2, strain for library amplification, strain for library excision) in LB medium to make the frozen stock? Does anybody has the pAS1 map in details? I could not find the selecting markers in the vector map. Thank you so much in advance. Regards Jianxiang Jianxiang Liu 1073 Roy J. Carver Co. Lab Department of Genetics, Development and Cell Biology Iowa State University Ames, Iowa, 50011-3650 Lab Tel: 515-294-4966 --- |
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| pas1 , pas1cyh2 , vector |
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