I got the library from ABRC for Y2H. In the kit there are four Ecoli strains.
Does anybody know which antibiotics I should add if I want to growth those
bacterial strains (pAS1, pAS1-CYH2, strain for library amplification, strain
for library excision) in LB medium to make the frozen stock? Does anybody has
the pAS1 map in details? I could not find the selecting markers in the vector
map. Thank you so much in advance.
1073 Roy J. Carver Co. Lab
Department of Genetics, Development and Cell Biology
Iowa State University
Ames, Iowa, 50011-3650
Lab Tel: 515-294-4966