Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Re Testing a Photobiotin labelled DNA probe. Method needed???

Re Testing a Photobiotin labelled DNA probe. Method needed??? - Protocols and Methods Forum

Re Testing a Photobiotin labelled DNA probe. Method needed??? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-10-2005, 11:16 PM
npercy
Guest
 
Posts: n/a
Default Re Testing a Photobiotin labelled DNA probe. Method needed???



Thanks for the reply Austin, sorry have to use google groups as the
newsserver doesn't seem to be functioning at the moment

You wrote
(i.e.

No filter on the lamp, there wasn't a lot of heat getting into the
icebucket in which the tube/DNA was contained. Not sure about the
liquid temprature but I held my hand there comfortably infront of it.
I had a look for what UV ranges the light should give out and it
should give a full range of visible, some infrared and longer wave UV.
I might have a look into trying another light thought not sure where
I will get it from (maybe use a transilluminator, but I thought that
would stuff up my DNA)

UV

The method I have is for a 500W lamp, I've seen 250W used also so
wasn't sure, these were using photobiotin activation with visible
light. Again I'm new to this so I'm still trying to find out about
it.

reaction.

Made up just before christmas, kept in a box, wrapped in Aluminimum
foil at -20C. I have minimised the light exposure where I can, so I
believe that this shouldn't be a problem

the
use

Yea, thats the problem I see for testing any probes I make, is the
fact that I could have a lot of loss through the system. I
unfortanetly dont have an achored system to test it with. The thought
I have been running is the idea of fixing the DNA to something and
then testing with my Strep-HRP congugate to see if I can get a
reaction. The problem with this is its extremely time consuming (a
day) just to see if the probe has worked, I also dont know whether its
not working due to other steps either (like the DNA sticking to the
membrane).

Thanks for the reply though

Nigel Percy
Reply With Quote
  #2  
Old 02-04-2005, 01:53 PM
Marco Herde
Guest
 
Posts: n/a
Default Re Testing a Photobiotin labelled DNA probe. Method needed???

npercy wrote:
Reply With Quote
  #3  
Old 02-04-2005, 02:05 PM
Marco Herde
Guest
 
Posts: n/a
Default Gateway-Transkription/Translation vector?

Dear All

I am looking for a vector for the in vitro transkription and translation
(like Promega´s TNT system) of a gene with a suitable leader etc.
I have cloned my gene in the gateway system, therfore I would prefer a
gateway-cassette in the vector.

Has anybody such a vector and would mind sending me an aliquot?

Thanks for your attention

Marco

--

Marco Herde
Albrecht-von-Haller-institute
Department for general and developmental plant physiology
Untere Karspüle 2
37073 Göttingen
GERMANY

Tel: 0551/3919365
Fax: 0551/397820

[Only registered users see links. ]
Reply With Quote
Reply

Tags
dna , labelled , method , needed , photobiotin , probe , testing


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Labelled Probe aftabac Protocols and Methods Forum 5 11-20-2006 05:04 AM
problems with probe binding to 28S and 18S RNA only on Northerns Wendy Ingram Protocols and Methods Forum 0 09-11-2006 02:30 AM
Testing a Photobiotin labelled DNA probe. Method needed??? npercy Protocols and Methods Forum 2 01-17-2005 02:04 PM
Product Testing Needed for Nanobiotech Separation Media Cindi Tepper Protocols and Methods Forum 0 10-21-2003 07:12 PM


All times are GMT. The time now is 11:10 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12375 seconds with 16 queries